For separating the non-volatile mixtures, a special chromatography technique is used, i.e. Thin Layer chromatography. It works on the principle of separation in which the different components are adsorbed on the surface of adsorbent such as silica gel, alumina or cellulose. It is carried out on a sheet or plate of glass, metal or plastic which is covered with thin layer of solid adsorbent material. This thin layer of adsorbent is also called as stationary phase. Usually, silica gel is used as an adsorbent with the size ranging from 400 to 800 meshes for tlc different binding grades including silica gel g, silica gel f, silica gel gf 254, silica gel hf 254. It can be used with or without binder. As a binder, Gypsum is used that has the ability to hold or stick the silica on the plates. When silica gel performs its task without binder, it performs fluorescence activity for easy analysis of compounds.
This chromatography technique performs several tasks that help in carrying out the separation process with highly positive outcome. These include observation of the evolution of a reaction, identification of the compounds present in a mixture as well as finding out the purity and cleanliness of a substance need to be obtained. This technique is considered as the cost-effective way to get simple, easy and faster results in the pharmaceutical and chemical industry where numbers of compounds are needed to purify or distil from the mixture. It is highly preferable mixture separation technique used in organic chemistry where reactions are qualitatively monitored.
The number of more improved methods can be used in order to enhance the resolution achieved with this thin layer chromatography. It also brings amazing results in getting more accurate quantitative analysis with high performance chromatographic separation. The main purpose of this chromatography method is to obtain well-defined and well-separated compounds from the mixture.
Sorbead India manufactures and supplies silica gel with or without different binder grade sizes which are effectively used in thin layer chromatography technique. You just need to select the most suitable silica gel grade size for separating the organic compounds as per the respective application. We ensure to offer economical products in bulk quantity to the worldwide clients by maintaining their effectiveness and quality.