Discovery of Chromatography technique has made a revolution in the field of analytical laboratories for the separation of the chemicals after synthesis. Since the separation process for each compound is different based on their physical interactions with the solute and the absorbent, different methods were discovered for an efficient and reliable analysis. Since for any chromatographic technique, the absorbent remains the same i.e. Silica Gel adsorbent, because it is low acidic and available in definite particle size ranging from 60-800 mesh size. There are many Chromatography techniques theoretically, but only few can be operated practically in the laboratories. Among these techniques Column chromatographic techniques are mostly preferred as it is low in cost as well as requires minimum instrumentation. In turn based on the flow of the solvent down the column through the absorbent, Column chromatography is classified into Gravity Column Chromatography and Flash Chromatography. The flow of the solvent in the column is based on the definite pore size of the Silica Gel, which allows smaller molecules into its matrix and thereby excludes the larger molecules which flow down and get eluted faster.
Silica Gel Mesh for Chromatography
If the solvent flows down the column passing the Silica gel absorbent of definite particle size by gravity or fissure, it is referred to as Gravity Column Chromatography. But if the solvent flows down the column passing the Silica gel absorbent of definite particle size by positive air pressure, it is referred to as Flash Chromatography. Therefore the absorbent used in both these techniques is the same, the Silica Gel Mesh. There are many products of Silica Gel mesh available depending on the mesh size such as Silica Gel 60-120 mesh, Silica Gel 60-200 mesh, Silica Gel 70-230 mesh, Silica Gel 200-400 mesh and Silica Gel 400-800 mesh. Among this Silica Gel 70-230 mesh is used for Gravity Column Chromatography and Silica Gel 200-400 mesh is used in Flash Chromatography. These all are available in a thin white free flowing powder form with a density of 0.75 gm per ml. Best separation is achieved in Ordinary compounds by Silica Gel Absorbent. Apart from Gravity Column Chromatography and Flash Chromatography, there is another technique in Chromatography named as Reversed-phase Chromatography, which is employed for the solvent which is more polar than the Absorbent. Silica gel was the first polymer used formerly in Reversed-phase Chromatography for the purification of small organic molecules but later for the purification of synthesized Peptides. The separation is achieved by the partitioning mechanism between the Stationary phase and the mobile phase in Reversed-phase Chromatography, in which the stationary phase is Silica Gel.
Overall, in each and every Chromatographic techniques the main absorbent is the Silica gel and is considered as the best absorbent in the analytical field, as it is porous, insoluble and its hydrophobic interaction with the solvent makes the elution process progress at a faster rate as the polar components get eluted first. These Silica gels exist in different particle sizes, because of which they are used in many Column Chromatography Techniques and Sorbead being the best Silica gel 200-400 supplier and Silica gel 70-230 mesh supplier.