Flash chromatography,also called as ‘medium pressure chromatography’, is performed with the mixture of two solvents, where one is polar component while other is non-polar component. This chromatographic separation process worksbyflowing down the solvent into the column with the help of external air pressure. It is carried out in order to separate the desired organic compounds from the unwanted compounds and other impurities. The solvent used for this type of chromatography depends on the retention factor. It provides higher percentage of desired natural product or compound as compared to conventional column chromatography due to the usage of high quality of silica gel and alumina particles 230-400 & 200-400 mesh size.
The smaller particles with higher mesh values are commonly used for flash chromatography. It is considered as the simple and economical way of separating the sample and solvent. For the purification of organic compounds, it acts as a fast and cost-effective technique with much better outcome of the desired compounds.It works using the plastic column filled with silica gel or alumina, and the sample that needs to be separated is placed on top of the selected adsorbent (silica gel or alumina). The rest of the column is filled with solvent. When air pressure is applied, the sample passes through the column and gets separated from the solvent. This way pure solvent or compounds are obtained.
It is used in wide range of applications that include purification of chemical compounds, industrial research laboratories, medical chemistry labs, separation of natural products, purification of proteins & vitamins and other pharmaceutical research applications.It is an ideal technique to meet the critical needs of the researchers in the industrial research labs.
Benefits of Flash Chromatography:
- Less solvent usage
- Greater flexibility
- Faster separation capacity
- Consistent purification performance
- High sample loading capacity
- High reproducible ability
Sorbead India provides bulk quantity of silica gel and aluminum oxide powder with different mesh sizes to be used for flash chromatography.We provide the best possible solution that ensures to suit your purification workflow needs. Our team of experts has designed the separation techniques to fulfill the requirements of various applications to obtain the desired end product.