Category Archives: Chromatography

RANGE OF SILICA GEL MESH FOR CHROMOTOGRAPHY

September 11, 2017


Discovery of Chromatography technique has made a revolution in the field of analytical laboratories for the separation of the chemicals after synthesis. Since the separation process for each compound is different based on their physical interactions with the solute and the absorbent, different methods were discovered for an efficient and reliable analysis. Since for any chromatographic technique, the absorbent remains the same i.e. Silica Gel adsorbent, because it is low acidic and available in definite particle size ranging from 60-800 mesh size. There are many Chromatography techniques theoretically, but only few can be operated practically in the laboratories. Among these techniques Column chromatographic techniques are mostly preferred as it is low in cost as well as requires minimum instrumentation. In turn based on the flow of the solvent down the column through the absorbent, Column chromatography is classified into Gravity Column Chromatography and Flash Chromatography. The flow of the solvent in the column is based on the definite pore size of the Silica Gel, which allows smaller molecules into its matrix and thereby excludes the larger molecules which flow down and get eluted faster.

 

Silica Gel Mesh for Chromatography

If the solvent flows down the column passing the Silica gel absorbent of definite particle size by gravity or fissure, it is referred to as Gravity Column Chromatography. But if the solvent flows down the column passing the Silica gel absorbent of definite particle size by positive air pressure, it is referred to as Flash Chromatography. Therefore the absorbent used in both these techniques is the same, the Silica Gel Mesh. There are many products of Silica Gel mesh available depending on the mesh size such as Silica Gel 60-120 mesh, Silica Gel 60-200 mesh, Silica Gel 70-230 mesh, Silica Gel 200-400 mesh and Silica Gel 400-800 mesh. Among this Silica Gel 70-230 mesh is used for Gravity Column Chromatography and Silica Gel 200-400 mesh is used in Flash Chromatography. These all are available in a thin white free flowing powder form with a density of 0.75 gm per ml. Best separation is achieved in Ordinary compounds by Silica Gel Absorbent. Apart from Gravity Column Chromatography and Flash Chromatography, there is another technique in Chromatography named as Reversed-phase Chromatography, which is employed for the solvent which is more polar than the Absorbent. Silica gel was the first polymer used formerly in Reversed-phase Chromatography for the purification of small organic molecules but later for the purification of synthesized Peptides. The separation is achieved by the partitioning mechanism between the Stationary phase and the mobile phase in Reversed-phase Chromatography, in which the stationary phase is Silica Gel.

 

Overall, in each and every Chromatographic techniques the main absorbent is the Silica gel and is considered as the best absorbent in the analytical field, as it is porous, insoluble and its hydrophobic interaction with the solvent makes the elution process progress at a faster rate as the polar components get eluted first. These Silica gels exist in different particle sizes, because of which they are used in many Column Chromatography Techniques and Sorbead being the best Silica gel 200-400 supplier and Silica gel 70-230 mesh supplier.

Use of Silica Gel in Flash Chromatography

Use of Silica Gel in Flash Chromatography

January 28, 2017


Flash Chromatography – An Introduction

Flash chromatography is also known as “medium pressure chromatography”. This form of chromatography works by air pressure driving the mixture, which is to be separated down the vertical glass column. This type of chromatography is ideal for separations that need to take place in a fast paced manner.

Continue reading

thin layer chromatography

Silica Gel Desiccants in Thin Layer Chromatography

September 27, 2016


Understanding Thin layer Chromatography

Thin layer chromatography is a widely used form of chromatography. This type of chromatography mainly finds use in the separation process of non-volatile mixtures. Thin-layer chromatography consists of both a stationary phase as well as a mobile phase. The stationary phase is usually an adsorbent like Silica Gel, Aluminium Oxide or Cellulose. The thin-layer chromatography process works by the molecular adsorbent like Silica Gel absorbing the different components of the mixture, which has to be separated, at different levels. The adsorbents for thin-layer chromatography are always decided in such a manner that they will quicken as well as improve the separation as well as the subsequent purification process.

Continue reading